竞博jbo亚洲第一电竞平台

回肠标准可消化组氨酸/赖氨酸对断奶仔猪(7-11kg)生长性能、肠道健康和含组氨酸蛋白动员的影响

发布单位:竞博jbo亚洲第一电竞平台

查看次数:2067

时间:2023-02-17
      试验目的在于评估高于NRC需要量的回肠标准可消化组氨酸/赖氨酸(SID H/L)对断奶仔猪(7-11kg)生长性能、肠道健康和含组氨酸蛋白(包括血红蛋白、肌肽和胰蛋白酶原)动员的影响。
      试验选用40 头断奶仔猪(26日龄;初重7.1 ± 0.5 kg),根据性别和初始体重的完全随机区组设计,分配至5种日粮处理。日粮中SID H/L分别为 26%、32%、38%、43% 和 49%,并饲喂14 天,日粮SID Lys含量为1.22%。在第0、7和 14 天分别记录采食量和体重。第 12 天采集血样。第14天对猪实施安乐死,采集胰腺、背最长肌、空肠中段和空肠粘膜样品。使用SAS的Proc Mixed对数据进行统计分析。
      日粮处理对仔猪生长性能没有显著影响,但是SID H/L显著影响血红蛋白水平(P<0.05,36%时浓度最高,为12 g/dL)、空肠粘膜中免疫球蛋白A水平(IgA,P<0.05,35%时浓度最低,为1.25 μg/mg),空肠中绒毛高度(P=0.065,40%时最大,为536 μm)、胰腺中胰蛋白酶原含量(P=0.083,41%时含量最高,为242 pg/mg)和肌肉中肌肽水平(P=0.051,38%时浓度最高,为4.7 ng/mg)。日粮SID H/L线性提高肌肉中的蛋白质羰基(P<0.05,从1.95到2.80 nmol/mg),并减少肠细胞增殖(P<0.05,从29.1%到26.9%)。
      总之,回肠标准可消化组氨酸/赖氨酸在35%-41%之间可以改善仔猪(7-11kg)肠道健康,并使含组氨酸蛋白水平达到峰值,这表明含组氨酸的蛋白质是确定组氨酸需要量时的有效标准。

The effects of standardized ileal digestible His to Lys ratio on growth performance, intestinal health, and mobilization of histidine-containing proteins in pigs at 7 to 11 kg body weight
The objectives were to evaluate the effects of standardized ileal digestible (SID) His:Lys ratio above the current NRC requirement on growth performance, intestinal health, and mobilization of His-containing proteins, including hemoglobin, carnosine, and trypsinogen, in nursery pigs from 7 to 11 kg body weight (BW). Forty pigs (26 d of age; initial BW of 7.1?±?0.5 kg) were allotted to 5 dietary treatments based on a randomized complete block design with sex and initial BW as blocks. Dietary treatments were supplemented with varying SID His to Lys ratios of 26%, 32%, 38%, 43%, and 49% and fed to pigs for 14 d (SID Lys?=?1.22%). Feed intake and BW were recorded at d 0, 7, and 14 to measure growth performance. Blood samples were collected on d 12. Pigs were euthanized on d 14 to collect pancreas, longissimus dorsi muscles, mid-jejunum, and jejunal mucosa. Data were analyzed using the Proc Mixed of SAS. Growth performance was not affected, whereas varying SID His to Lys ratio affected hemoglobin (P?<?0.05, max: 12 g/dL at 36%), immunoglobulin A (IgA, P?<?0.05, min: 1.25 μg/mg at 35%) in jejunal mucosa, villus height (P?=?0.065, max: 536 μm at 40%) in jejunum, trypsinogen (P?=?0.083, max: 242 pg/mg at 41%) in pancreas, and carnosine (P?=?0.051, max: 4.7 ng/mg at 38%) in muscles. Varying SID His to Lys ratios linearly increased (P?<?0.05, from 1.95 to 2.80 nmol/mg) protein carbonyl in muscles and decreased (P?<?0.05, from 29.1% to 26.9%) enterocyte proliferation. In conclusion, SID His to Lys ratio between 35% and 41% in diets fed to nursery pigs at 7 to 11 kg enhanced intestinal health and maximized concentrations of His-containing proteins, indicating that His-containing proteins are effective response criteria when determining His requirement.

文章来源:猪营养国际论坛
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